DNA Extraction and Purification
Macherey-Nagel NucleoBond Xtra Maxi EF, 50 preps
NucleoBond Xtra Maxi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A
INTACT GENOMICS INC RNA Cleanup Kit 25 Preps
The Intact Genomics IG RNA Cleanup Kit purifies up to 50 µg of RNA. Has a rapid and simplified workflow with a single wash buffer. Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt;)Option to Elute in 20-50 µL for more concentrated RNA than the standard 100 µL; Standard 70-100% RNA recovery; Customizable buffer options for difficult reactions; Purified RNA is ready for use in a wide variety of downstream applications including: RT-PCR, RNA library prep for NGS, formation of RNP complexes for genome editing, microinjection, RNA labeling, and transfection.This kit is recommended for use following the IG sgRNA Synthesis Kits to remove proteins, salts and most unincorporated nucleotides. The Intact Genomics RNA Cleanup Kit has a simplified workflow and is ideal for cleanup and concentration of RNA after enzymatic treatments such as DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT).
New England Biolabs, Inc. Monarch® gDNA Wash Buffer - 60 ml
Monarch gDNA Wash Buffer is a component of the Monarch Genomic DNA Purification Kit. It is an ethanol-based, low salt buffer that allows for complete removal of chaotropic salt and cell components in just 2 wash steps, enabling the purification of highly-pure gDNA.
- A component of the Monarch Genomic DNA Purification Kit
- An ethanol-based, low salt buffer that allows for removal of chaotropic salt and cell components
- Supplied as a concentrate
- Monarch kit components are also available separately for added convenience
Abcam DNA Isolation Kit - Plasma/Serum, 50 Tests
DNA Isolation Kit - Plasma/Serum (ab156893) is a complete set of essential components which enables the experimenter to efficiently isolate DNA from plasma, serum, and body fluids.
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. Monarch® gDNA Blood Lysis Buffer - 20 ml
The Monarch gDNA Blood Lysis Buffer contains a strong protective component against the high nuclease activity in blood samples and supports rapid degradation of hemoglobin and other protein components. This buffer is a component of the Monarch Genomic DNA Purification Kit.
- A component of the Monarch Genomic DNA Purification Kit
- Supports rapid degradation of hemoglobin and other protein components
- Contains a strong protective component against the high nuclease activity in blood samples
- Monarch kit components are also available separately for added convenience
Bioland Scientific LLC Plasmid Miniprep Column, 250 columns, DNA-binding matrix, 10-30 μg plasmid DNA, 1-5 mL culture, less than 20 min processing time
The Bioland Scientific Plasmid Miniprep Column (250 count) is designed for the fast and efficient purification of high-purity plasmid DNA from bacterial cultures. Utilizing a DNA-binding specific matrix, these spin columns facilitate the isolation of 10-30 μg of high-copy plasmid DNA from 1-5 mL of culture in under 20 minutes. The purified DNA is suitable for various downstream molecular biology applications.
- Column type: Spin column
- Quantity: 250 columns
- Capacity: 10-30 μg plasmid DNA
- Input culture volume: 1-5 mL bacterial culture
- Purification principle: DNA-binding specific matrix
- Processing time: Less than 20 min
- Elution compatibility: Tris buffer or pure water
- Downstream applications: Restriction digestions, PCR, automated sequencing, transfection, restriction mapping, library screening, gene therapy, genetic vaccinations
Macherey-Nagel NucleoSpin Microbial DNA, 10 preps
NucleoSpin Microbial DNA (10) 10 preps for the isolation of DNA from microorganisms - NucleoSpin Bead Tube Type B, NucleoSpin Microbial DNA Columns, Collection Tubes, buffers
Biotium RNase-Free Calf Thymus DNA, 1 mg/mL (1 mL)
During isolation of DNA, RNase treatment is performed to eliminate contaminating RNA. Because RNase activity is robust and resistant to denaturation, commercial preparations of DNA contain high levels of residual RNase activity, even after re-purification. Preparing DNA solutions in DEPC-treated water will not inactivate RNase already present in the DNA. Similarly, commercial RNase inhibitor additives are usually poorly effective, expensive, and interfere in downstream applications. Biotium has developed a unique proprietary method that irreversibly inactivates RNases in purified DNA. The resulting DNA solution is free of excess protein or other chemicals that may interfere with downstream applications. Our RNase-free Calf Thymus DNA solution undergoes stringent quality control testing to confirm the product is 100% free of RNase activity. The solution is provided as 1 mg/mL calf thymus DNA in TE. Store the product at 4⁰C.
Biotium AccuGreen™ High Sensitivity dsDNA Quantitation Kit (100 assays)
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA. The assay has a green fluorescence with an excitation/emission of 502/523 nm (with DNA). The assay has a linear range of 0.1-100 ng dsDNA and a sample concentration range between 5 pg/uL and 100 ng/uL. This trial size kit has sufficient reagent for 100 assays.
Zymo Research Corporation Quick-DNA™ Viral 96 Kit (2 x 96 Preps)
Quick recovery of viral DNA from a wide range of sources using Zymo-Spin plate technology. Plate design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is suitable for PCR, Southern blotting, and restriction endonuclease digestion.
Zymo Research Corporation Quick-DNA HMW MagBead Kit (96 prep)
Innovative reagents and Zymo Researchs unique system allow for a simple Bind, Wash, & Elute procedure that is unmatched in providing ultra-pure and high yield DNA of > 150 kb size. Isolated DNA is ready for immediate use in sensitive downstream applications including long read (Oxford Nanopore™) and NGS sequencing, qPCR, arrays, and methylation analysis.
Zymo Research Corporation Zyppy™ Elution Buffer (5 ml)
Zyppy Elution Buffer is designed to be used with our Zyppy Plasmid Miniprep Kit, which uses a pellet-free modified alkaline lysis method to isolate ultra-pure plasmid DNA from E. coli in only 8 minutes.